Dorothy, and her big city lawyer boyfriend, return to the Lazy 'B' ranch to read her late father's will. For Dorothy to inherit everything, she must stay on the ranch for 5 years. If she does not, everything goes to Buck, who is the manager. She does not like Buck, so she makes a deal with the wrong people for cattle and then the outlaws go to the ranch to get the $10,000 from her. But Buck is on the job.
When the showboat hits town, two men use the parade as a distraction to rob the bank. Their accomplice is Pop, the clown from the showboat. He leaves the money on the boat and tells his daughter Patsy to bring it to him at a later stop on the river. Gene's investigation of a bank robbery takes him to the showboat where he becomes a performer. Gene and Frog try to find the money while helping Patsy and her father.
Tom "Killer" Dane kills Jeff's friend, who then pursues him. Jeff and Dane are look-alike half brothers, which allows Dane to make a raid dressed like Jeff. Jeff is arrested, but before Dane's henchman can organize a lynch mob, Fuzzy breaks him out and Jeff heads after Dane again.
Vignettes weaving together the stories of six individuals in the old West at the end of the Civil War. Following the tales of a sharp-shooting songster, a wannabe bank robber, two weary traveling performers, a lone gold prospector, a woman traveling the West to an uncertain future, and a motley crew of strangers undertaking a carriage ride.
Peterson has a plan to obtain all the ranches in the valley. He gives Carson a phony Spanish land grant and has him pose as the Mexican owner. When Fred and Fuzzy have their cattle stolen by Peterson's men, they quickly become involved in the scheme.
Gene is the foreman at the ranch owned by wealthy rodeo owner Maureen. She will lose her rodeo contract unless sales improve.
Tex and his sidekicks arrive to help out his friend Jeffers, a railroad owner, only to find that he has been killed. They quickly run into trouble with an outlaw gang in their attempt to find the mysterious ghost train that supposedly runs on Jeffer's line.
A singing cowboy roams the Wild West with his sidekick, dancing horse and fancy wardrobe.
James Kincaid controls the local water supply and plans to do away with the other ranchers. Government agent Sandy Saunders arrives undercover to investigate Kincaid's land swindle scheme, and win the heart of one of his victims, Fay Denton.
Ranger Eddie Dean is looking for the outlaw the Tioga Kid, a man he closely resembles. He runs into Joe Morino and his gang of rustlers at the same time Tioga arrives to cut himself into Morino's game. But Morino doesn't give in and in the showdown, Eddie and the Kid find themselves on the same side.
Eddie Dean's assignment is to thwart the efforts of a crooked gambler, Brad Barton, to take over the property of his half-brother Bill Ryan. In order to secure the ranch, which is believed to hold large silver deposits, the scheming relative contracts to have Ryan killed. He then presents a forged will to the court naming himself as the sole heir. Shocked by the tide of events, Ryan's two rightful heirs, his grown daughter Robin and young son "Freckles" are determined to remain on their father's property. Eddie and his sidekick, Soapy Jones, arrive on the scene in time to enter the fight on the side of Robin and "Freckles."
A Hollywood singing-cowboy star with a big heart and an even bigger secret (he uses a double in most scenes because he can't ride, fight or sing) comes to the aid of a rancher about to lose his home on a rodeo bet.
Kate Diamond owns the Roaring Falls Trading Post from where she directs her gang's gun-smuggling to the Indians. After she short-changes smuggler Stacey, his men attempt to steal the hidden guns, and attack her foreman Nebraska, but he is saved by Jimmy and "Cannonball" on their way to file a homestead claim at Canyon City. Jimmy renews a long acquaintance with Sheriff Harris and his daughter Jessica. The sheriff is wounded by half-breed Danny when he finds a rifle hidden in the latter's wagon, but Jimmy captures the outlaw, a go-between for Kate and the Indians.Wounded and in bed, Sheriff Harris ask Jimmy not to tell Jessica that she is only adopted and that Nebraska is really her father, although he believes her to be dead.
When asked about the Ghost Riders song he sings, Gene Autry tells this legend: Gene is about to resign as an investigator for the county attorney and go into the cattle business with his pal Chuckawalla Jones but decides instead to help Anne Lawson clear her father, rancher Ralph Lawson, of a false murder charge. He looks for the three witnesses who can testify that Lawson shot only in self defense in killing a gambler, but the witnesses are terrorized by another gambler, town boss Rock McCleary, who shoots witness Pop Roberts Morgan. Fatally wounded, Pop gives Gene the information needed to clear Lawson, then dies crying the "Ghost Riders" are coming for him. Gene then heads for a showdown with McCleary.
Marshal Landry captures outlaw Girard and bringing him in finds a woman and two children, the only survivors of an Indian attack. Later, transferring the prisoner his brothers free him. Then a stage is robbed of a silver shipment by Girard and his brothers. Examining telegrams gets Landry a confession from Girard's girlfriend. The telegraph line has been tapped and the telegrapher is the supposedly dead husband of the woman he brough in. Now knowing Girard's location he sets out after him.
Gene Autry becomes the new Sheriff after bank robbers kill the former sheriff. When Judge Beaumont is murdered, evidence points to the judge's wild son. Believing the young man, Gene tries to help.
Caxton has broken out of prison and Eddie has been sent to bring him in. Caxton is known by the polka dot band on his hat and Eddie has Soapy wear one like it. This gets Soapy arrested as soon as he rides into town but it leads Eddie to Varney and he realizes Varney will lead him to Caxton.
A singing cowboy (Dick Foran) thwarts a thieving judge and courts a woman (Anne Nagel) in Texas.
As war looms in the Pacific, even cowboys are called on to fight the enemy. Horses are desperately needed by U.S forces stationed in the Philippines, and the Range Busters - Crash Corrigan, Alibi Terhune, and their newest partner, Davy Sharpe - are hired to accompany a herd to the islands. But first they must battle Axis spies right in Texas, as they foil a plot to steal the very horses destined for shipment.
Roy returns home to fine a range feud between the cattlemen and the sheepmen. When his friend is killed he finds the rifle had a defective pin. He learns the rifle belongs to a ranch hand named Barker and that a third party has caused the feud. When he captures outlaws trying to blow up a dam, he claims Barker was the killer. But Barker has switched rifles and the outlaws now accuse Roy and Roy finds himself in trouble.