After the Texas Rangers are disbanded, the the reconstruction years following the Civil War, a private state-police force extorts money from the citizens in a "protection" scheme. Ex-Rangers Jimmy Wakely and "Cannonball" Taylor foil an arrest by state-police officers Hamon and Kelly. Commissioner Jed Brant tells his nephew that his old friend Jimmy is plotting against the law-and-order forces. Vic's fiancée and ranch-owner, Sheila Carol, refuses to sign up with the crooked police outfit, and believes Jimmy is an outlaw. On Chief Barton's order, Hamon shoots ex-Rangers Murphy and Payson, so they can be blamed for a raid on Sheila's ranch.
Gene and Frog set out to find out who has been causing the accidents at a dam construction site.
Americans come west to California in the hope of peaceful settlement. Roy and Gabby sing a duet: "We're Not Coming Out Tonight." Other songs include "Sundown on the Rangeland" and "Ride on Vaquero."
It's cattlemen versus sheepmen and Trigger Gargan appears to be the leader of the gang causing the trouble. But unknown to Ranger Tex Lawrence, the respected town citizen Barrow is the boss and is tipping off the gang as to the Ranger's activities.
Ranch owner Sandra, fresh from animal husbandry school, brings a flock of sheep into cattle country. The local ranchers don't like it, and ranch foreman Gene must deal with it.
Jimmy Wakely and his sidekick "Lasses" White run into trouble as they attempt to hire some cattle cars on the Cattleman's Railroad to take their herd to market. Rancher Joseph Colton has bought up all the cattle cars and intends to purchase the penniless line from principal stockholder Gywnn Randall. She is eager to sell to Colton but doesn't realize that he intends to force all the ranchers out of business once he has control of the line.
Kate Diamond owns the Roaring Falls Trading Post from where she directs her gang's gun-smuggling to the Indians. After she short-changes smuggler Stacey, his men attempt to steal the hidden guns, and attack her foreman Nebraska, but he is saved by Jimmy and "Cannonball" on their way to file a homestead claim at Canyon City. Jimmy renews a long acquaintance with Sheriff Harris and his daughter Jessica. The sheriff is wounded by half-breed Danny when he finds a rifle hidden in the latter's wagon, but Jimmy captures the outlaw, a go-between for Kate and the Indians.Wounded and in bed, Sheriff Harris ask Jimmy not to tell Jessica that she is only adopted and that Nebraska is really her father, although he believes her to be dead.
Jimmy Wakely wishes to sell to the government a group of wild mustangs which he has captured and trained for rancher Matt Blake. The competition is Flora Carter, the owner of a near-by ranch. The army colonel in charge decides to buy the winning horses of a cross-country race. Flora has her henchmen, led by Sam Phelps resort to foul means in order to try to win the race.
Hughie Mack, a not so nice western singer, is discovered by Clover Doyle as the next movie cowboy hero. His name is changed to Slim Carter and a promotional buildup begins. Leo Gallaher, an orphan boy wins the contest to spend a month with Slim. Leo is a good influence on his cowboy hero. Clover sees the good and more in Slim. Montana Burriss is Slim's double.
Roy is a newspaper reporter. He goes to Cheyenne to cover the activities of supposed bad guy Arapahoe Brown. Roy, of course, discovers who the real bad guy is.
Diamonds are being smuggled across the border from Mexico in a specially made shoe of a palomino mare. One of the smugglers is killed when the mare runs off. The sheriff blames Trigger for the death. To keep his horse from being destroyed, Roy confesses and goes to jail. The smugglers buy Trigger and put him to work smuggling diamonds. The mare, who had earlier heard a trist with Trigger, foals Trigger, Jr. who Roy, finally out of jail, uses to help capture the smugglers.
Tom is a cowboy boot-wearing cat at a Texas dude ranch. When a beautiful female cat comes for a visit, Tom takes time from his regular torturing of Jerry to use the mouse as a way to impress the dame. Naturally, Jerry gives Tom his comeuppance.
Bob Blake and his sidekick and four singing cowboys arrive at the Jackson ranch where Bob learns from Betty Jackson that her brother, Joe, is missing. Bob investigates and learns that there is gold on the Jackson ranch, and the neighboring rancher has kidnapped Joe in order to get his land.
A cowboy is wrongfully accused of murder. He winds up in Harlem, where he assumes the identity of a preacher-turned-gangster who looks like him. He infiltrates the gang to catch the men who framed him.
In his starring debut, Roy gets elected to Congress in order to bring water to the ranchers in his district. In Washington, he learns he needs the backing of a key congressman and gets that man to go west for an inspection trip. When the congressman is initially unimpressed, Roy gets the inspection party stranded without water to show the true conditions.
As the sheriff of a small western town, Autry sings his way into a relationship with Eleanor, a singer from a Chicago nightclub who earlier witnessed a murder.
If a young lady gives up her inheritance the local ranchers will lose their free grazing land.
Melody arrives looking for the killer of his uncle and at the same time Dumont arrives looking for the murderer of her father. They both suspect Skelton and Dumont finds incriminating evidence in his office. But when Melody finds the murder weapon in Skelton's office he is arrested by Shelton's stooge Sheriff.
When it appears that Fred Jamison is a member of Red's gang, he is kicked out of the Rangers. But it's just a plot between Fred and the Ranger Captain. Fred then gets into Red's gang and makes plans that will enable the Rangers to bring them all in. But his message to the Captain is intercepted and the hoax revealed.
Young Joe is paralyzed as he is bucked by a wild horse, a strawberry roan. Angered, his father, Walt, tries to shoot the horse but is stopped by his foreman, Gene Autry. The roan escapes and Autry, told to leave the ranch by Walt, finds and trains the horse, now named Champ, in hopes that by returning it to Joe it will provide him with the will to overcome his disability.