Eddie Dean's assignment is to thwart the efforts of a crooked gambler, Brad Barton, to take over the property of his half-brother Bill Ryan. In order to secure the ranch, which is believed to hold large silver deposits, the scheming relative contracts to have Ryan killed. He then presents a forged will to the court naming himself as the sole heir. Shocked by the tide of events, Ryan's two rightful heirs, his grown daughter Robin and young son "Freckles" are determined to remain on their father's property. Eddie and his sidekick, Soapy Jones, arrive on the scene in time to enter the fight on the side of Robin and "Freckles."
Finding Indians stealing from his ranch, Gene learns they are suffering from malnutrition. Store owner Martin is cheating them and now he is after the Chief's valuable necklace. When the dying chief is found, having been attacked and robbed, Martin blames Lakhona who would become the new chief. When Gene helps Lakhona they soon find themselves fleeing from the law.
Eddie Dean (Eddie Dean) and his partner Soapy Jones (Roscoe Ates), under government orders, proceed to the ranch of J.C. Morgan (Shirley Patterson to buy cavalry remounts for the Army. At the ranch, they find out that J.C. is a girl. The nearby town of Dow City is under the control of a lawless trio headed by Trigger (Lee Roberts, Clem Kensington (Foxy Callahan) and Joe Morino (Dennis Moore). A member of the gang is Tucson Brown (Lee Bennett), one of J.C.'s trusted hands. When Eddie decides to buy the horses, Tucson steals the herd to prevent the sale. Soldiers, sent to investigate, are brutally murdered. The aroused townspeople elect Tennessee (William Fawcett, J.C.'s foreman, as sheriff. When the outlaws murder Tennessee, Eddie and Soapy, along with the reformed Tucson, swing into action.
A singing cowboy and his sidekick rescue miners from a bad deal.
A greedy rancher is charging excessive prices for access to the area's only water supply, extorting the smaller ranchers in the area. A water diviner teams up with a cattle buyer to force the villain to share the water with his neighbors.
President Lincoln personally sends Bill Gibson west to see if he can stop the holdups of the needed shipments of gold. There he meets his boyhood friend Foster. When all others refuse to take out the next gold shipment due to the killings, Bill volunteers. Jeannie, afraid for his safety, tells Foster of Bill's secret route not knowing Foster is the leader of the outlaw gang.
A Hollywood singing-cowboy star with a big heart and an even bigger secret (he uses a double in most scenes because he can't ride, fight or sing) comes to the aid of a rancher about to lose his home on a rodeo bet.
Ranger Eddie Dean is looking for the outlaw the Tioga Kid, a man he closely resembles. He runs into Joe Morino and his gang of rustlers at the same time Tioga arrives to cut himself into Morino's game. But Morino doesn't give in and in the showdown, Eddie and the Kid find themselves on the same side.
Near Border Flats, Don Coyote and his friend Sancho are interrupted on their way to the fiesta by a fight. A quick intervention on their part prompts ranch owner Maggie Riley to hire them. Coyote and Sancho meet her surly, younger brother Ted who is wanting Maggie to sell their cattle herd to pay off a bank loan before they lose the ranch. But when they try to drive a herd to market, a gang led by Big Foot Ferguson drives off their cowhands.
With the Government Remount Service unable to meet it's quota of horses, Marshal Fred Martin arrives to try and find the rustlers. Apparently just a singing cowhand dressed in white, he becomes the masked Two Gun Troubador dressed in black when he goes after the outlaws. He quickly learns Tex Walters is the leader of the gang but he must find out who is the big boss that gives Walters his orders.
The fourth of 12 singing Westerns starring the "Silvery-Voiced Baritone," Fred Scott, Melody of the Plains begins peacefully enough with Scott, as cowboy Steve Condon, warbling Don Swander and June Hershey's "Albuquerque." The story quickly takes a rather grim turn when one of Steve's colleagues is shot and killed after selling out to a gang of rustlers. Mistakenly believing he fired the deadly shot, a dejected Steve, along with sidekick Fuzzy, goes to work for Bud's father, a rancher nearly forced into bankruptcy by a crooked land developer.
Tex and his sidekicks arrive to help out his friend Jeffers, a railroad owner, only to find that he has been killed. They quickly run into trouble with an outlaw gang in their attempt to find the mysterious ghost train that supposedly runs on Jeffer's line.
Gene is the foreman at the ranch owned by wealthy rodeo owner Maureen. She will lose her rodeo contract unless sales improve.
Sandy Doyle, gambler and political chief of a small border town, seeks to gain control of the Bar-X Ranch, owned by Rufe Rickson, to further some undercover activities of his own. He counts on Rickson's inability to stay away from gambling as the means to his ultimate success. Government investigator Oliver Shea and his assistant, Dan Haggerty, start a fight in Doyle's place when they see Rickson being cheated and are invited to the Bar-X where Oliver and Helen Rickson, Rufe's daughter, discover interest in each other and Dan finds himself pursued by Bell, the ranch cook. Sheriff Larson brings the prize money for the $5,000 race of the Rodeo Association, and that night it is stolen.
In the midst of the Civil War, Lassiter has a plan to get control of California. Working out of St. Joseph, he plans to send forged messages to the troops on the west coast via Pony Express. First he attempts to bribe Pony Express ride Roy Rogers. When Roy refuses he turns to the outlaw Johnson and his gang and this leads to trouble.
Peterson has a plan to obtain all the ranches in the valley. He gives Carson a phony Spanish land grant and has him pose as the Mexican owner. When Fred and Fuzzy have their cattle stolen by Peterson's men, they quickly become involved in the scheme.
His horse Champion steals the show from Gene when what's at stake is a horse race and a bull fight.
Gene Autry hunts bank robbers Al Bartlett and Trot Lucas with his old friend Mike. Bartlett, to throw off his pursuers, kills Trot and his own brother. When Kitty Bartlett comes to town claiming to be the slain Bartlett's widow, Gene has to save her from the irate townspeople who are not aware that her name isn't Bartlett but she really is the daughter of a law officer slain by Al Bartlett. Ben Luder, a local hood, tricks Bartlett back into town by saying he has to fixed to have Doc Larry Taylor do plastic surgery on him. En route they meet Doc and his assistant Helen Ellis and Ben's ruse is exposed. Bartlett kills Ben and forces Doc to drive him to the railroad. Gene, in a fight atop a runaway train, captures Bartlett.
Mining executive Neal Wallace arrives to investigate the losses at a gold mine and is immediately framed for murder. The murderers then incite a lynch mob but the Sheriff lets him go. Wallace eventually convinces the Sheriff of his innocence and the two then work together to get the gang that is looting the gold ore.
When Tasker kills Roy Rogers he takes one of his young sons. Fifteen years later the other son Roy arrives buying a ranch in the valley where Tasker now controls the water supply. Roy organizes the ranchers for a showdown with Tasker not knowing that his brother is Tasker's chief henchman.