While the Civil War rages on between the Union and the Confederacy, three men – a quiet loner, a ruthless hitman, and a Mexican bandit – comb the American Southwest in search of a strongbox containing $200,000 in stolen gold.
Mining executive Neal Wallace arrives to investigate the losses at a gold mine and is immediately framed for murder. The murderers then incite a lynch mob but the Sheriff lets him go. Wallace eventually convinces the Sheriff of his innocence and the two then work together to get the gang that is looting the gold ore.
A greedy rancher is charging excessive prices for access to the area's only water supply, extorting the smaller ranchers in the area. A water diviner teams up with a cattle buyer to force the villain to share the water with his neighbors.
Wild Bill Hickock and Cannonball help two young people in love and bring the murderer of Cannonball's father to justice.
A renowned former army scout is hired by ranchers to hunt down rustlers but finds himself on trial for the murder of a boy when he carries out his job too well. Tom Horn finds that the simple skills he knows are of no help in dealing with the ambitions of ranchers and corrupt officials as progress marches over him and the old west.
When Tasker kills Roy Rogers he takes one of his young sons. Fifteen years later the other son Roy arrives buying a ranch in the valley where Tasker now controls the water supply. Roy organizes the ranchers for a showdown with Tasker not knowing that his brother is Tasker's chief henchman.
Gene Autry hunts bank robbers Al Bartlett and Trot Lucas with his old friend Mike. Bartlett, to throw off his pursuers, kills Trot and his own brother. When Kitty Bartlett comes to town claiming to be the slain Bartlett's widow, Gene has to save her from the irate townspeople who are not aware that her name isn't Bartlett but she really is the daughter of a law officer slain by Al Bartlett. Ben Luder, a local hood, tricks Bartlett back into town by saying he has to fixed to have Doc Larry Taylor do plastic surgery on him. En route they meet Doc and his assistant Helen Ellis and Ben's ruse is exposed. Bartlett kills Ben and forces Doc to drive him to the railroad. Gene, in a fight atop a runaway train, captures Bartlett.
Roy returns home to fine a range feud between the cattlemen and the sheepmen. When his friend is killed he finds the rifle had a defective pin. He learns the rifle belongs to a ranch hand named Barker and that a third party has caused the feud. When he captures outlaws trying to blow up a dam, he claims Barker was the killer. But Barker has switched rifles and the outlaws now accuse Roy and Roy finds himself in trouble.
His horse Champion steals the show from Gene when what's at stake is a horse race and a bull fight.
A former gunslinger is forced to take up arms again when he and his cattle crew are threatened by a corrupt lawman.
With the Government Remount Service unable to meet it's quota of horses, Marshal Fred Martin arrives to try and find the rustlers. Apparently just a singing cowhand dressed in white, he becomes the masked Two Gun Troubador dressed in black when he goes after the outlaws. He quickly learns Tex Walters is the leader of the gang but he must find out who is the big boss that gives Walters his orders.
The fourth of 12 singing Westerns starring the "Silvery-Voiced Baritone," Fred Scott, Melody of the Plains begins peacefully enough with Scott, as cowboy Steve Condon, warbling Don Swander and June Hershey's "Albuquerque." The story quickly takes a rather grim turn when one of Steve's colleagues is shot and killed after selling out to a gang of rustlers. Mistakenly believing he fired the deadly shot, a dejected Steve, along with sidekick Fuzzy, goes to work for Bud's father, a rancher nearly forced into bankruptcy by a crooked land developer.
Sandy Doyle, gambler and political chief of a small border town, seeks to gain control of the Bar-X Ranch, owned by Rufe Rickson, to further some undercover activities of his own. He counts on Rickson's inability to stay away from gambling as the means to his ultimate success. Government investigator Oliver Shea and his assistant, Dan Haggerty, start a fight in Doyle's place when they see Rickson being cheated and are invited to the Bar-X where Oliver and Helen Rickson, Rufe's daughter, discover interest in each other and Dan finds himself pursued by Bell, the ranch cook. Sheriff Larson brings the prize money for the $5,000 race of the Rodeo Association, and that night it is stolen.
Atkins is the boss of one of the Pony Express relay stations. He has been causing trouble and is replaced with Cal Sheridan. Atkins now gets the Richard brothers to raid one of the relay stations and they kill Norma's father. Cal sees that the horse of one of the raiders has a broken shoe and Norma sets out to find that horse.
In the midst of the Civil War, Lassiter has a plan to get control of California. Working out of St. Joseph, he plans to send forged messages to the troops on the west coast via Pony Express. First he attempts to bribe Pony Express ride Roy Rogers. When Roy refuses he turns to the outlaw Johnson and his gang and this leads to trouble.
In the old west, a man becomes a Sheriff just for the pay, figuring he can decamp if things get tough.
Gene is the foreman at the ranch owned by wealthy rodeo owner Maureen. She will lose her rodeo contract unless sales improve.
Tex and his sidekicks arrive to help out his friend Jeffers, a railroad owner, only to find that he has been killed. They quickly run into trouble with an outlaw gang in their attempt to find the mysterious ghost train that supposedly runs on Jeffer's line.
Peterson has a plan to obtain all the ranches in the valley. He gives Carson a phony Spanish land grant and has him pose as the Mexican owner. When Fred and Fuzzy have their cattle stolen by Peterson's men, they quickly become involved in the scheme.
A young boy journeys to the old west to help the local sheriff defend his town from a villainous gang of outlaws.