Hoppy and new sidekick California Carlson head to California to help out Lucky Jenkins.
Buck Peters arranges for Hoppy, California, Johnny and other cowboys to go to the aid of friends whose cattle are being rustled. Hoppy and California locate the rustlers' hideout and join the gang by posing as outlaws themselves, but must find a way to let the rest of the posse know where they are.
Hoppy, California and Lucky arrive at a remote inn, where Lucky expects to be married - but finds the bride-to-be in distress over her uncle, who has suddenly disappeared from the inn. Then Hoppy finds the uncle's body in the shaft of his nearby mine...
Hoppy, California and Johnny partner up with brother and sister ranch owners, two of several who are having their access to water blocked by a dam owned by a greedy merchant in town, who is intent on driving them out and taking their land for himself.
One of Hoppy's Bar 20 ranch hands is tricked into participating in nefarious activities after being subjected to hypnosis.
Posing as a cattle buyer, Hoppy crosses over into Oklahoma where the Jordan brother's and their outlaw gang operate outside the law. After receiving an unfriendly reception when he finds them, he, California, and Johnny rustle their cattle and drive across the river into Texas. He hopes they will cross over to retrieve their cattle and then he can arrest them.
Hoppy is leading a scientific expedition and the Chinese who have a hidden settlement nearby are trying to stop them. Saulters and his outlaw gang are also in the area looking for a gold mine. When Saulters men attack, the gold mine is found. Hoppy agrees to file for the Chinese and heads after Saulters in the chase to the land office.
Hoppy's ranch is threatened by rustlers. Hoppy and the gang oblige as usual.
Hoppy, California, and Lucky travel to Mesa City for a short vacation. California buys new clothes and carrying his old ones in a suitcase, bumps into escaping bank robbers in the dark. His suitcase gets switched with that of the robbers and he is seen with the money. Hoppy must find the money that has mysteriously disappeared and also the robbers so that he can clear California who is now in jail.
A young Eastern lawyer, seriously injured in a stage holdup, secures the help of Hoppy, California and Jimmy in completing his mission to his woman cousin's ranch in Texas. The ranch, as are others in the same area, is being plagued by a gang called the Night Riders, while the friendly local town lawyer is trying to cajole the cousin into selling out to him. Hoppy begins by arriving in the town, separate from his pals, all spiffed up and dandified, posing as the Eastern lawyer...
Hoppy goes undercover as a gambler from the East when Bar 20 cattle are stolen by unknown rustlers. Brennan/Talbot are twin brothers (one a casino owner, the other a rancher) and Hoppy believes they provide alibis for each other while one is out committing crimes. Hoppy gets a job in the casino to learn more but is exposed when a gambling gunslinger notices him.
U.S. Marshal Hopalong Cassidy is called when a town becomes overun with bad guys. Disguised as a member of a medicine show, Hoppy discovers that the ringleader is none other than sweet li'l ol' Ma Burton.
An evil gang is involved in both cattle rustling and the robbing of stagecoaches. Hoppy must stop them without help from the sheriff who turns out be a major outlaw himself.
A crusading newspaper editor recruits his old friend Hoppy to take the job of Marshall in a town rife with vice and murder directed at helpless miners.
On a cattle drive Hoppy, camp cook Windy, companion Lucky, and young Artie Peters encounter an eccentric professor. The professor professes to be searching for the evolutionary missing link, but in reality he is a cattle rustler who uses his dynamite to scatter the cattle in order capture some of them. Hoppy and Bar 20 guys ultimately capture the professor.
Hoppy and his pals arrive in a remote town to investigate the counterfeiting of both U.S. and Mexican money; his only clues are the name "Mordigan" and a drawing of a comet. He quickly finds out that Mordigan is the town "boss"; but what or who is "the comet", and why are Mordigan and his henchmen intent on persecuting a young woman, her drunken brother, and her deathly ill sister-in-law who've also just arrived in town?
Hoppy, California and Johnny come to the ranch of a friend and his daughter, disguised as dude detectives from the east, to investigate the disappearances, without a trace, of several herds of cattle.
Hoppy's brother has been murdered and he is on the trail of the murderers. To get them he makes himself seem to be a wanted man.
Problems come in the form of one of Hopalong Cassidy's neighbors, but the matter is settled when Hoppy roots out the troublemaker.
The U.S. Army needs more horses for the Spanish-American War. Hoppy must turn his Bar 20 cowhands into Rough Riders to gather up the horses, and of course bad guys try to sabotage the operation.